Mon, September 13, 2021

Highly sensitive analysis of latex agglutination (antigen-antibody reaction)

Our paper entitled “Latex agglutination analysis by novel ultrasound scattering techniques” by Kana Kitao and Tomohisa Norisuye has been accepted for publication in Ultrasonics.

Do you think that ultrasound is suitable for emulsified samples and concentrated systems, but weak for dilute systems?

In this paper, we proposed a new method that is neither averaging nor frequency filtering to remove unwanted signals (noise) and detect reactions with high sensitivity. A specific application is the latex agglutination method, in which antigen is added to a suspension of particles with antibodies on them, and the antigen-antibody reaction is observed via agglutination of the particles.

While the advantage of the ultrasonic scattering method is that it can access concentrated systems up to several tens of wt%, there is a problem that the accuracy becomes inaccurate below several wt%. In this study, latex agglutination can now be detected in a dilute system of 0.625 μg/mL, and since 1 mg/mL is about 0.1 wt%, it is possible to analyze concentrations of less than 1/1000 of that level, which is quite low for ultrasound.

However, if we pursue only high-sensitivity detection, there are other excellent methods, such as light scattering and QCM methods, which have reached 1 ng/mL sensitivity. Ultrasound technology needs to work harder, but it is also important to think of unique ways to utilize ultrasound based on newly obtained findings.

posted at September 13, 2021 10:44 PM

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Polymer Molecular Engineering Laboratory
Department of Polymer Science and Engineering,
Kyoto Institute of Technology,
Matsugasaki, Sakyo-ku, Kyoto 606-8585, JAPAN